A few years ago, we sought to develop a generic, highly efficient approach for isolation of intracellular bacterial pathogens.
The paper summarizes results of using "dilution" approach for isolation of active sites in metal oxide catalysts.
We demonstrate a systematic approach for isolation and validation of microsatellites for the Cx.
Meanwhile, we established an approach for isolation and enrichment of CSCs in vitro from human Panc-1 cell lines.
The FISH and related linkage map results followed known reinforced the relationships of homoeologous chromosomes in allotetraploid cotton, and presented a useful approach for isolation of homoeologous loci or segments and for mapping of monomorphic loci.
The original approach for isolation of exosomes, still widely used, is based on ultracentrifugation in combination with sucrose density gradients or sucrose cushions to float the relatively low-density exosomes away from other vesicles and particles [ 31].
Currently the most popular approach for isolation of exosomes is based on ultracentrifugation [ 31], which allows the researcher to obtain highly pure exosomes; however, it is a very lengthy, difficult, and unreliable process.
In this study, we applied shotgun 454 pyrosequencing, which is a more efficient approach for isolation of microsatellites at a fraction of the cost and effort compared to traditional Sanger methods (Zalapa et al., 2012), to develop 19 nuclear microsatellite markers for P. deflexa.
A traditional microbiological approach for isolation and identification usually takes 2 to 3 days, and given that different numerical profiles for Y. ruckeri can be obtained with commercial multi-substrate identification systems, particularly the API 20E system, they must be interpreted with caution [ 3].
The long-term goal will be to use a similar approach for isolation and characterization of additional cell wall/cell surface components that will allow not only comparisons of cell wall composition between related algae but also between cell walls of land plants and the algae from which they were evolutionarily derived.
