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In pursuing comparative genomics approaches for functional inference of protein function, the initial selection of related proteins separated by great evolutionary distances can be a challenge.
Synthetic promoters and introns provide useful approaches for functional validation of promoter sequences.
The application of this genome-editing technology to hematology has afforded new approaches for functional genomics and even the prospect of "correcting" dysfunctional HSCs in the treatment of serious genetic hematological diseases.
Our approach delineates the exon/intron structure necessary to extract the protein coding sequence for experimental and homology based approaches for functional interrogation.
Experimental approaches for functional characterization of gene products are often compromised by incorrect or insufficient knowledge of the gene structure to enable extraction of the protein sequence for experimental studies.
Both of these approaches support accurate structural annotation which is a requirement for experimental approaches for functional characterization and also provides validated sequence to utilize tools that enable identification cellular localization signals, domains.
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Besides, the combination of multiple methods in this study can be used as a general approach for functional studies of a protein with unknown function.
The authors propose a new approach for functional safety risk analysis.
Our work thus provides a useful approach for functional reconstitution and engineering of fungal CODSs for efficient production of this family of anticancer molecules.
The results demonstrated a general principle for the creation and application of this RNAi library approach for functional gene discovery within a predefined protein family.
A unified approach for functional task formulation is developed that allows communication between user and team of mechatronic engineers developing the mechatronic handling devices for requested task function.
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