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At best, this approach was optimized by prior genetic studies (whole-genome association studies (WGAS) or whole-genome homozygosity mapping (WGHM)) to restrict the list of candidates in case of consanguineous families and/or large series of patients.
Nevertheless, we are sure that our study lacks a significant number of nTARs as our approach was optimized for higher specificity to reduce the number of false positives opposed to higher sensitivity that would result in a minimal number of false negatives.
For quinic acid bearing molecules, the approach was optimized for the detection of m/z 191 [quinic acid-H+] and a similar method was used for the other molecules herein.
The Sanger sequencing approach was optimized, automated and employed to decode the 3 billion base human genome reference sequence via the Human Genome Project.
It should be noted that some of the minor bile acid species present in the gut luminal samples may not have been detected, as the analytical non-targeted LC-MS approach was optimized to detect as many metabolites as possible and not targeted specifically toward bile acids.
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A key benefit of the PAD approach was optimizing the tablet interface.
In general, the Bayesian and random search approach were optimized very quickly (in less than 20 iterations), whereas the grid search method required many more iterations before the SVM reached optimal performance: 57 iterations (the lowest number) were required for EstateFP, and 138 (the highest number) for MACCSFP).
The combination of all 3 different approaches was optimized to a workflow that produces a reliable structural model, useful for a meaningful analysis of ligand receptor interactions, not seen in the original model.
These approaches are optimized in the field of pulmonary rehabilitation which is the reference therapy of COPD-associated undernutrition.
These two compounds belonging to different chemical classes were chosen for the optimization of the method considering that the approach developed was optimized with respect to the radioligand binding assay.
Although the SBE approach offers improved scale, performance was optimized mainly to discriminate between homozygosity and heterozygosity for the purpose of high throughput genotyping.
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