Sentence examples for approach using restriction from inspiring English sources

A cloning approach using restriction endonucleases LguI and/or Eco81I enabled a step-by-step continuous ligation of two fragments in vector pQE-30-LE.

Miller et al. [ 12] developed a rapid and cost-effective polymorphism identification and genotyping approach using restriction site-associated DNA (RAD) markers.

Conventional 4C approaches used restriction enzyme digestion to fragment chromatin, and recently sonication approach was also applied for this purpose.

GBS is a reduced representation approach that uses restriction enzymes to sample the genome.

DNA methylation is one of the best studied epigenetic marks and can be assayed genome-wide using restriction enzyme, bisulphite or enrichment-based approaches (reviewed in Laird, 2010).

One approach would be to work with large blocks of DNA carved from wild M. genitalium using restriction enzymes.

GBS and similar techniques such as RAD-Seq [ 6] are reduced representation approaches that use restriction enzymes to target the sequencing effort to a fraction of the genome.

This approach used a restriction enzyme and a size selection step to generate and sequence a defined fraction of a large genome.

Unlike the RAD-seq, this approach uses two restriction enzyme comprising a rare-cutting one and frequently-cutting one and avoids random shearing of the DNA.

We developed an approach to estimate R Z A using restriction-site associated DNA RAD; [ 19] markers sequenced on the Illumina platform.

Zeschnigk et al. applied a similar approach using 454-sequencing with a combination of 4 different restriction enzymes [26].