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Use of artificial myocardial tissues might be a promising approach to reconstitute degenerated or failing cardiac tissue in many disease states and therefore provide a reasonable alternative to whole organ transplantation.
The inherent flexibilities with abundance of polar contacts provide an efficient approach to reconstitute protein trans-splicing.
Reviewer 1: The authors take a bottom up approach to reconstitute the oscillatory motion of the MinCDE system.
This positioning of FtsZ in these micro compartments is another step in the bottom up approach to reconstitute bacterial cell division.
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To more carefully characterize the requirement for RIPK3, we next used genetic approaches to reconstitute RIPK3 expression in the RIPK-deficient, death-resistant HEY cells and to suppress RIPK3 expression in the death-inducible OVCAR3 cells.
Here, we describe approaches we developed to reconstitute the human Fe S cluster assembly machinery in Escherichia coli and to define its remarkable architecture.
Currently, the inability to expand an efficient HBV-specific immunity in a large proportion of adult CHB patients (particularly those with high HBV replication levels) [ 9] might suggest that more radical approaches are necessary to reconstitute a functional efficient HBV-specific T-cell repertoire.
With this approach, we were able to reconstitute a time course of mRNA changes related to the cell cycle and observed a sharp peak at an unexpected early time point, namely P9.5, during which a large cluster of genes related to the cell cycle was upregulated.
To study the biochemical roles of lysine methylation, several approaches have been developed to reconstitute chromatin fibers in vitro with well-defined methylation patterns.
One novel approach to this problem has been to reconstitute RyR1 with fluorescently tagged RyR binding proteins, FKBP12 and calmodulin, and then to measure FRET between these proteins bound to RyR1 [9].
In this study, a simple and green approach 'bioinspired disassembly-reassembly strategy' was employed to reconstitute lipoprotein nanoparticles (RLNs) using whole-components of endogenous ones (contained dehydrated human lipids and native apolipoproteins).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com