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In order to shed light on which regions of the Munc18-1 protein are involved in regulation of exocytosis independent of closed conformation syntaxin binding we undertook an unbiased structural-functional approach to insert 15 base pair linkers randomly into the Munc18-1 coding sequence.
The third model used a gene-targeting or KI (knock-in) approach to insert a chimaeric mouse/human exon 1 containing 140 CAG repeats into the murine huntingtin homologue (Hdh) and thus expresses the mutant HD gene in the normal context of the mouse genome (Menalled et al., 2003).
To selectively inhibit the γ-tubulin complex in the larval epidermis, we used a CRISPR/Cas9-based approach to insert a sequence encoding GFP downstream of the gip-2 gene at its endogenous locus on Chr I and introduced the 'epiDEG' transgene encoding a fusion of an anti-GFP nanobody with ZIF-1, which recruits the target to a Cullin2-based ubiquitin ligase, on Chr II.
It was not a product so much as an approach to insert intelligence across the Salesforce platform.
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The most obvious approach is to insert more pipeline registers in the critical path in order to increase the system clock.
However, the catalytic effects of rGO can be enhanced with doping; one such approach is to insert cations or anions, but we need to be mindful of the costs of the fabricated catalyst as well as its efficiency [21 24].
The most promising current approach is to insert a functional gene into the patient that will lead to the production of either the native protein or a compatible non-immunogenic equivalent version.
An alternative approach is to insert the lesion into a double-stranded vector such that the damaged and nondamaged strands are marked by distinct sequence signatures.
Another approach was to insert more copies of the TAKA amylase gene under the TAKA promoter into strain CF1.1 which already has elevated protein expression.
The posterior approach and the anterolateral transgluteal (ALT) approach, also called the direct lateral approach, without trochanteric osteotomy are with various variations probably the 2 most common approaches used to insert a total hip arthroplasty (THA).
A knee approach was made to insert the K-wire in the femur, followed by the trochanteric approach to pull out and bend the K-wire.
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CEO of Professional Science Editing for Scientists @ prosciediting.com