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With regard to a rational approach of virus attenuation, we consider a selective deletion of viral genes that modulate the immune response of the host.
The potency of this fusion inhibitor is based on its ability to block the conformational change of gp41 and to avert the approach of virus and cell membrane.
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In the clinical setting, one potential approach of the virus would be used in a two-step procedure: the virus would be applied to the surgical wound at the time of resection followed by multiple post-resection I-iodine PET scans (at two- to four-month intervals).
We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS).
During this outbreak of SARS, we have applied this combination approach of conventional virus isolation and molecular detection to establish the viral infection status of other patients hospitalized for SARS.
A more distant but highly exciting prospect is the development of autonomous devices having functions approaching that of virus particles (e.g., cell entry via attachment and fusion) or primitive immune cells (e.g., input-based therapeutic response), and which could provide significant advances in diagnostics and therapeutics.
This combinatorial genetic approach of combining viruses with genetically altered mice is eminently suitable for targeting cell type specific gene expression in select brain subregions.
In addition, the approach of discovering viruses infecting plant or animal hosts by directly examining insect vectors that feed upon them is directly applicable to other host-vector systems (e.g., mosquitoes and human viruses; Ng et al., in review).
In view of these considerations and in line with the accepted taxonomic approach to viruses of the family Coronaviridae [ 3], comparative sequence analysis was considered the most reliable basis for classification.
Altogether, engineered resistance has expanded the scope of innovative approaches for virus control by providing new tools to develop resistant crop cultivars and increasing opportunities to implement effective and sustainable management strategies.
Overall, our results indicated that the combination approach of using conventional virus isolation and molecular detection could be successfully applied to the isolation of HMPV.
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