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To identify ZNF191 target genes, a combined approach of transient overexpression and transient knockdown (KD) are used to identify genes modified by the ZNF191 transcription factor with 44 K Whole-Genome 60-mer oligonucleotide microarrays, using a cellular model (HEK293).
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Since a bioinformatic evolutionary comparison analysis (phylogenic footprinting) did not reveal significant sequence conservation between the 5'-regions, we utilized an experimental approach consisting of transient transfection of luciferase reporter constructs in four distinct cell lines and ex vivo electroporation of mouse retinal explants with GFP reporter plasmids.
In this Note, we present a new numerical approach of the transient flow problem through homogeneous porous medium (say dam).
This paper presents a novel modeling approach of coupling transient computational fluid dynamics (CFD) simulation with system identification for control system involving fluid flow and heat transfer.
The approach of coupled transient thermal analysis to account for the temperature fields generated during the dwell and deposition phases of the process resulted in reasonably accurate results, with an error of 18% between absolute maximums.
The first objective of this study was to evaluate the feasibility of an agro-infiltration approach for transient expression of a T-DNA construct in leaf tissues of rice.
research, online simplified nonlinear control approach for transient stabilization enhancement of multi-machine power systems is considered, whereas in Haotian et al.'s research, switching excitation control approach for enhancement of transient stability of such systems is investigated [10, 11].
The approach enables calculations of transient molecular deformation coefficients in entire range of elongation rates and times.
Besides the traditional approaches, like transfections of transient expression plasmids, transduction of adeno-associated viral (AAV) [ 130], lenti-, retror or adeno-viral vectors, new ways to deliver the gene or protein cargo to the cells have been discovered.
The magnetoelectric composite was fabricated by bonding a magnetostrictive layer to a piezoelectric layer using a novel approach of low temperature transient liquid phase (LTTLP) bonding.
A validated simulation was used to visualize on the one hand the heat path as isothermals and on the other hand to show an alternative approach of the electrical transient correction.
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