The near-sigmoid model approach permits estimation of replication start timing T0 and replication end timing T100 at various loci of the genome.
With respect to the direct scoring approach the utility of replication groups does not reduce false positive rates, and may, depending on the data set, often perform worse.
We ultimately settled on the very robust, but more labor intensive, approach of manual plate replication by spotting of single colonies in two sets of ordered arrays.
We need diversity of approaches, replication of findings, innovation and even some incrementalism.
Although much of the data generated to date has been produced from unreplicated designs, this approach can accommodate various levels of replication and provides a consistent platform for making activity calls.
Two main approaches to replication can be distinguished: master slave and multi-master replication.
Combining genomic and genetic approaches together with the analysis of replication intermediates, we searched for factors coordinating replication with transcription.
We employed an integrated knowledge translation approach to conduct a concept analysis of replication research.
Taken together, the in silico approach can predict the putative origin of replication of most bacterial species closely related to Wolbachia including Ehrlichia, Anaplasma, and Rickettsia.
