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We report the architecture of S. cerevisiae INO80 using an integrative approach of electron microscopy, crosslinking and mass spectrometry.
To identify the second interface needed for lattice formation and to test its function in viral RNA synthesis, we used a hybrid approach of electron microscopic and biochemical evaluation of both wild-type and mutant viral polymerases to evaluate computationally generated models of this second interface.
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These are circumstances under which projection maps alone are easily misinterpreted and our approach of combining electron tomography with electron crystallography enables a straightforward interpretation (Gerle et al., 2006; Tani et al., 2013).
Using a combined approach of immunofluorescence, electron microscopy and biochemistry we have followed and characterized the internalization of mouse PrP (moPrP) transfected in FRT (Fischer Rat Thyroid) cells that do not express cav-1 and do not have caveolae (FRT-PrPC), as well as in FRT cav-1 cells (FRT-PrPC/cav-1) which form caveolae [45].
Utilizing computational approach, distribution of electrons within the molecules has been explored.
In a real experiment or 3D approach, scattering of electrons due to the transverse force of wakefield increases final electron bunch emittance and is close to the true value.
According to thermodynamic approach, the direction of electron flow will occur from higher chemical potential to the lower electronic chemical potential, until the electronic chemical potentials become equal.
Due to the very large number of total Rubisco catalytic sites the overall rates of carbon dioxide fixation approach maximum rates of electron transfer.
Moreover, the incorporation of F atoms into complex 3 (3b, 3b) can be efficient approach of tuning the electron injection ability, balance of charge transfer process, and emitting color.
A new computational tool to represent microbial pathways through microbial metabolic reactions (MMRs) using the approach of the Thermodynamic Electron Equivalents Model has been designed and implemented in the open-access framework NetLogo.
In recent debates, Steinborn[ 5] and Loock[ 6] advocate Pauling's[ 7] approach of assigning shared electron pairs to the more electronegative atom.
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