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The nonderivatized glycomics approach implemented here can be performed in relatively mild conditions, with reduced sample handling, and subsequently will produce glycan profiles with minimal perturbations.
Overall, the methodological approach implemented here may have broad application for estimating kinship patterns in systems with limited genealogical information and/or in studying similarly challenging organisms in which obtaining biological materials from complete families is problematic.
Because our methodology is based on general acoustic features found in most animal vocalizations, we suggest that the approach implemented here could fruitfully be applied to other vocal taxa.
Accordingly, the spatial statistical approach implemented here may be useful for other countries where HCV prevalence is considerable, such as Cameroon, Congo, Gabon, Guinea, Malawi, Mongolia, Pakistan, and Romania.
From a methodological standpoint, the approach implemented here, although not free from limitations, can have broad application for analyzing systems with limited genealogical information and/or in studying similarly challenging organisms in which obtaining genetic data on complete families is problematic.
The approach implemented here, a simple motif search for the conserved ends of 59-be sites identifies gene cassettes with almost 100% efficiency, provided that sequence information is available from gene cassettes of an integron belonging to the same class as the target.
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Space time approaches implemented here represent a tool that can provide information on an important public-health metric at an accuracy that is likely to be useful to decision makers.
Thus, only the one-vs-all approach was implemented here.
The ML approach, as implemented here, incorporates information about inbreeding F into the model [ 5].
However, to date, they have not yet been directly demonstrated using a systematic analysis approach, as implemented here.
Interestingly, feature extraction using the Bayes Feature Extraction approach as implemented here is intuitively similar to the application of position-specific scoring matrix (PSSM) profiles for predicting protective linear B-cell epitopes by EL-Manzalawy et al.[ 21].
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