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Nucleic acids amplification-based techniques proved to be the most rapid and reliable approach for pathogen diagnostics and identification.
Here we introduce a novel approach for pathogen detection using short reads, generated by deep sequencing of short RNA extracts.
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For instance, clinical diagnosis and the epidemiological study of microbial strain circulation will be profoundly remodeled in the near future by the use of HTS, which should, very soon, be used as a characterization approach for pathogens and which will probably slowly replace the present PCR and biochemical based characterization methods [ 13, 14].
Development of rapid molecular approaches for pathogen detection is key to improving treatment of infectious diseases.
With VIZIONS, we are aiming to integrate traditional clinical, epidemiological, and medical anthropological methods with new approaches for pathogen detection and discovery, including novel sequencing approaches combined with phylogenetic analysis to characterize pathogen populations.
In this work, we have developed a simple microfluidic approach for pathogenic microorganism detection (not just pathogen DNA).
Although these methods are being improved for incredibly greater sensitivity [ 1- 3], the efficiency of any diagnostic approach for direct pathogen assessment depends upon the presence of agent in a small specimen matrix.
Using a new software approach for identifying pathogens, he and his colleagues have identified an association of Fusobacterium species with colorectal carcinoma.
To discover endogenous small RNA species in Bcp in response to Ecc infection, we developed a highly efficient approach for cloning pathogen-regulated small RNAs.
MS-based proteomics provided an unrestricted and unbiased approach for surveying pathogens in honey bee colonies.
Broad-range rDNA polymerase chain reaction (PCR) provides an alternative, cultivation-independent approach for identifying pathogens.
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