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Genomic DNA from Pa ATCC43949 was sequenced by a shotgun approach and sequences were assembled using the Phusion assembler [ 18], based on read pair information.
The nuclear loci consisted primarily of intronic regions with primers designed using an exon-primed intron spanning approach and sequences from the chicken and zebra finch genome (following [ 46]).
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To identify the responsible mutations, we used a candidate approach and sequenced the coding sequences of several connexin genes.
Therefore, we took a candidate gene approach and sequenced the exonic regions of Cbr-lin-39 in bh20 animals.
The present study provides a methodologic approach and sequence information to carry out a large-scale screening of mating-type loci organization in Z. sapae and Z. rouxii.
Thus, by combining both text mining candidate selection approach and sequence-based classifier, a novel hybrid strategy is proposed for gene priority with a specific function protein.
In addition, one 5 ng bulk RNA as a control was amplified by a tube-based approach and sequenced into both SE50 (8 million reads) and PE91 (46 million reads).
The choice between a whole-genome shotgun sequencing approach and sequencing reduced representation libraries was extensively discussed during the Human Genome Project (Lander et al., 2001; Venter et al., 2001), and the former became the dominant technology as the sequencing throughput rapidly increased, rendering library techniques to prepare data for the latter approach relatively expensive.
The dorsolateral PFC is responsible for planning approaches and sequences of behaviour that are required for goal-directed behaviours.
Clayton-Smith et al identified KAT6B, a histone acetyltransferase, as the causative gene for SBBS by a whole-exome sequencing approach and reported sequence variants in 13 individuals.
Thus, operative approaches and sequencing of surgical repair are guided by imaging information displayed by CT.
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