Main effort of this work is focused on applying, to each involved parameter, the standard framework for uncertainty evaluation as prescribed by the Guide to the expression of uncertainty in measurement.
Mouse serum was diluted 1∶10000, and 100 µl of the diluted samples were applied to each well along with the standard, incubated at 37 °C for 1 h, washed 3 times with PBS-Tween, and then incubated with 100 µl of the biotinylated monoclonal antibody (2 µg/ml) for another 2 h.
Before meta-analysis, genomic control (GC) was applied to each study by multiplying the standard error of the effect estimates by the square root of the study-specific inflation factor (λ = 1.04 in SF, λ = 1.03 in SCALE).
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Compared with cell image analysis, flow cytometry demonstrated a significantly higher proportion of diploid tumours, which may be related to differences in the internal standards applied to each method.
Additionally, 10 microlitres of each of 6 standard mixtures, which contained increasing concentrations of corresponding lipid standards, were applied to each plate.
Cell culture sample or recombinant standard were applied to each well and allowed to incubate for 6 h in a humidified atmosphere of 5% CO2.
In each run, a total of 48 samples were analysed, including one standard sample applied to each run, one negative control, one repeat within the runs and several other repeats (altogether 5%%).
In all the studies, whilst a formal pathological evaluation was undertaken, different standards were applied to each study and overall comparison was difficult to make.
Draft specific standards and guidelines that apply to each procedure.
For calculation of mRNA expression levels, Ct values per gene were applied to standard curves, generated for each gene of interest.
