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Applying this challenge dose of both pathogens in SM10 resulted in a strong activation of the bovine TLR2 receptor in the HEK293 reconstitution system of TLR-signalling (Additional file 1: Figure S1A), similarly as previously reported [ 9].
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Most of the organizations in the process of applying this technology encounter with challenges and may have failure despite of spending a lot of energy and huge investments.
However, there are significant uncertainties associated with time-dependent lateral flow rates, which creates a challenge when applying this method.
The challenge of applying this approach to the central nervous system (CNS) is that we often do not know the details of what is needed from the biological side.
The main challenge in applying this procedure is the identification of a valid instrument.
The challenge in applying this method for probing oxidative stress in corals is to define whether these extracellular fluxes reflect intracellular processes.
The main challenge in applying this methodology to different settings is the integration of technical constraints with meaningful scenarios.
The challenge of applying this methodology in radiochemistry is the design, synthesis, and purification of the required precursor.
The rapid accumulation of molecular data poses the clinical challenge of applying this data in a timely manner in clinical trials.
A further challenge to applying this clinical evidence-base to clinical training is that the techniques and terminology used in the field have been poorly standardized.
A challenge in applying this model to a programme change such as the ETA is identifying the appropriate level at which to consider the influences of the political climate (the politics stream).
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CEO of Professional Science Editing for Scientists @ prosciediting.com