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The matrix solution (saturated sinapinic acid in 0.5% trifluoroacetic acid/50% acetonitrile) was applied to each spot twice and then the ProteinChip arrays were analyzed by the ProteinChip reader Series 4000 (Bio-Rad), applying the reading protocols optimized for low and high mass to charge (m/z) range.
The concentrations were determined after applying the reading to the linear part of the standard curve generated by the NADH standard supplied.
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We applied the read depth methodology [ 26, 34, 35] to estimate CNVRs.
The concentration was calculated by applying the sample reading to a standard curve.
This can be achieved by applying the theory of reading the intensity of gel electrophoresis bands by using densitometry analysis software.
This may be caused by applying the test or reading the test results under the wrong conditions.
As each seed structure is processed, a hash table is constructed to index all the reads based on the result of applying the structure to the read sequences.
Raw data were processed by means of the analysis pipeline for whole genome shotgun sequence reads applying the GS FLX System Software (version 2.6).
"We deeply regret that this incident occurred, and we are undertaking a thorough review of our public safety officers' actions, and will address our processes and procedures and how they are applied," the statement read.
We then applied the super-reads reduction using a database of 79-mers to the deep-coverage paired-end data from the megagametophyte.
If you don't know how to properly apply the gel, read the instructions that come along with it.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com