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Before applying samples onto the gel, 10 min of preelectrophoresis (40 W at 35°C) was performed.
When the proposed strategy is applied, i.e., applying samples in twofold or fourfold and supplementing them with one of the confirmatory solutions penicillinase, 4-aminobenzoate, or magnesium sulfate, the test allows presumptive group identification and initial screening in one step.
In the new experiments reported on in this revised version, we combine an enzyme (apo-PP1, PP1+R15 or PP1+R15+G-actin) and a substrate (eIF2αP or PYGMP) at various concentrations and we measure the rate at which the substrate is converted to product over time by applying samples of the reaction mix at different time points to a PhosTag gel that separates the substrate and product.
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Unequal selection probabilities are compensated by applying sampling weights.
We extracted total RNA from lung homogenates and applied samples for realtime RT-PCR.
Adjacent skin without any applied samples or occlusion served as control.
In some cases, automation of sampling (Multivial cyclone) was then augmented by automated processing steps to apply samples to biosensors (MVI).
Because the median filtering is applied sample per sample, it results in "noisy" temporal curves.
When each sampling strategy was applied, sample sizes were gradually reduced and each sampling size contained 3000 replicates.
Blood is routinely sampled from laboratory animals in biomedical research, and many of the commonly applied sampling techniques require anesthesia.
The first approach is to apply sample average approximation to approximate the two stochastic problem via sampling.
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