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By applying length, %GC and conservation thresholds we have significantly enriched our list of IGRs for known structured RNAs.
OMACC is advantageous because it takes into account gap size carefully via rescaling optical map and applying length constraint on selecting the path of contigs for gap closure.
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Raw sequencing data were processed with the ACGT-miR-v3.5 package (LC Sciences) to filter out adaptors, technical impurities, repetitive sequences, and low-quantity reads (<3) while applying length-cutoff values (15 26 nt), resulting in a nonredundant sequence list.
In principle, we can apply length dependent rescaling factors.
Specifically, it rescales optical map and applies length constraint for selecting the correct contig sequences for gap closure.
More importantly, it applies length constraints, uniqueness of contig alignment, and a loop-path recording method to avoid deep recursion.
For IMEx pattern search, we used the previously applied length threshold parameters (Mono-, 12; Di-, 6; Tri-, 4; and for Tetra- to Hexa- repeats, a minimum stretch of three minimum repetitions) (9).
Take a long strip and apply length-wise, securing the front and back.
Moreover, the combination of applying unbonded length and additional vertical ties could postpone the rebar buckling in SP1-R and SP3-R specimens, and subsequently the rebar rupture.
SSFA is applied to length profiles (length-scale analysis) and to three-dimensional surfaces (area-scale and volume-filling scale analyses).
Because we could obtain the best F-measure for both X and Y datasets by applying the length-normalized edit distance, we considered the weighted version of the length-normalized edit distance.
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