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In the course of establishing several Ecotilling projects, screening up to 12 kb in more than to 1500 samples, we developed a streamlined procedure for setting up the experiment, dealing with the results and interpret the possible functions of newly discovered SNPs widely by applying freely available software tools (figure 4).
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The word laughingstock was applied freely, and why not?
It took a while for people to catch his deadpan sense of humor, which he applied freely to himself.
In a world today where words such as "courage" and "heroism" have been so overused -- applied freely from sports to entertainment to politics -- as to be rendered practically meaningless, Jan Karski was the rare human being who embodied both.
In addition to applying existing freely available software tools, new scripts have also been developed, e.g. to produce coverage plots, which show the number of reads overlapping each position in the reference genome for easy quality assessment, and to convert SAMtools output files into HaploGrep input files for convenient haplotype calling.
He applied paint freely and loosely with the brush and sometimes reworked it with his fingers.
Over the years, that idea has been applied more freely, to all manner of quartets and even to chamber music in general.
Rather than apply paint freely to contrast the orderly color fields, he often snakes twisting but hard-edge lines through them.
Modern neuroscience and the interest in biomimetic control design demand increasingly sophisticated experimental techniques that can be applied in freely moving animals under realistic behavioral conditions.
The resulting algorithm is thoroughly validated by several numerical tests and finally applied to freely rising bubbles with stationary and oscillatory shape as well.
I prefer the stripped-down version, and can't help wishing that the principle had been applied more freely in the past: "Bad & Beautiful," "Good, Bad & Ugly," "Remains of Day".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com