Exact(1)
However, by applying culture dependent and independent approaches [ 18, 20, 58], more diverse communities including bacteria from the Alpha-, Beta-, Gamma-, anDeltaproteobacteriaia subclasses were revealed in similar ecosystems like the Inner Mongolian Soda Lake [ 17] and the hyperalkaline spring waters in Jordan [ 67].
Similar(58)
This makes it necessary to A) consider the bias factor when applying culture-medium-based predictive models to sausage; B) apply the dynamic version (described by differential equations) of those models; C) combine growth and death models in a continuous way, including the highly uncertain growth/no growth range separating the two regions.
Although PCR amplification with gene-specific primers of total DNA extracted from MBC had shown the presence of several AR genes, when applying culture-dependent approaches to isolate the corresponding AR strains, we were able to identify AR colonies only from the raw materials employed for cheese production, in which microbial titers are higher than in the final product [ 13].
Under the applied culture conditions, P. cinnabarinus SBUG-M 1044 produced an extracellular phenol oxidase with an activity of around 0.5 U mL-1.
Nevertheless, the applied culture methods have been adapted to isolate the common bacteria known to be involved in bronchial infections of CF patients.
These data demonstrate a differentiation of pmATII cells towards an ATI-cell-like phenotype under the applied culture conditions.
To investigate the composition of organisms in this space, we applied culture-independent approaches to microbes harvested from the air of two densely populated urban buildings, from which we analyzed 80 megabases genomic DNA sequence and 6000 16S rDNA clones.
The applied culturing method proved to be suitable for the purpose of the current study.
To improve the reproducibility of high-throughput cultivation processes and the comparability between different applied cultures, it is strongly recommended to use automated or manual liquid handling stations or, alternatively, multi-channel pipettes.
This study showed that the conventional method of using 96-pin replicators to transfer E. coli PR02-cells from one MTP to another MTP can result in highly variable growth kinetics of the applied cultures.
Some of the removals currently taking place are doubtless appropriate, but many likely spring from applying one culture's standards to another.
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