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To circumvent most of these difficulties, emphasis has been placed on cell culture methodologies to study growth regulation, hormonal responsiveness, or biochemical properties of mammary epithelial cells (MEC).
A novel cell culture methodology is described in which diffusion tensor magnetic resonance imaging (DTMRI) and cell micropatterning are combined to fabricate cell monolayers that replicate realistic cross-sectional tissue structure.
The latter studies have been hampered greatly by the absence of facile cell culture methodology for this clinically important virus [17], [47].
Standard cell culture methodology was employed (Fan et al, 1998, 2001a, 2001b).
DP, KL and PD performed the immunohistochemistry. DM participated in the study design and established the cell culture methodology.
In addition, in vitro propagation of CSCs applying stem cell culture conditions has been repeatedly reported.
Collagen is a well-known universal cell binder and is widely applied in cell culture [23].
Treatment: Treatment applied in cell culture (none = no treatment).
The method was applied to cell culture material originating from bench and pilot scales.
Notably, SILAC labeling has been most frequently applied to cell culture or microorganisms.
A strategy of combining MJA elicitation and sucrose feeding was applied to cell cultures in bioreactors.
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