To solve this problem, the DEA applies a secondary goal.
Specific for EPA 314.1 is the necessity to apply a secondary confirmation column with different chromatographic selectivity to validate the perchlorate results obtained with the first separator column.
After this step, we applied a secondary criterion to check individual quality for each CNV.
After applying a biotinylated secondary antibody and tertiary reagent (Vector-Laboratories, Bulingam, CA, USA) the antibody binding was visualised by diaminobenzidine (Serva, Heidelberg, Germany) and H2O2.
Among those applying for a secondary school place this time round is the prime minister's daughter Nancy Cameron, who is expected to go to a central London girls' comprehensive.
So if you're thinking of applying to a secondary campus, don't write it off completely – just be aware that it may not provide the full university experience that you were hoping for.
Speculating that this may have been due to mis-annotated base-pairs in RFAM (which applies a consensus secondary structure to every sequence in an alignment), we next used the mix80 dataset, where each sequence is individually annotated with its own structure.
Immunoreactivity was detected using the ABC method (Vector Labs, Burlingame, CA, USA) or by applying a suitable fluorescence-labelled secondary antibody.
This colour, achieved by grinding semi-precious stone, was always applied as a secondary, a secco stage, along with the gold leaf applied as haloes and decoration to robes.
The primary antibody was detected by applying a horse anti-rabbit HRP secondary antibody and an avidin-biotin complex (Vector Laboratories, Burlingame, CA) followed by diaminobenzidine.
