Sentence examples for applying a gradient of from inspiring English sources

Urea was removed by applying a gradient of 200 ml using 0.2 M NaH2PO4, 1 M NaCl, pH 4.2.

The recombinant protein was eluted by applying a gradient of 0 to 1 M NaCl over 10 column volumes.

This solution was loaded onto a 5 ml HisTrap HP (Amersham Biosciences, Buckinghamshire, United Kingdom) and the bound proteins were eluted by applying a gradient of 50 to 500 mM imidazole.

Red squares show the case in which no temperature gradient is presented, and blue circles show the effect of applying a gradient of temperature in our experiment.

Separation of various XOS was achieved by applying a gradient of NaOAc (5 to 85 mM) in 150 mM NaOH over 30 min at 30°C, using a flow rate of 1 ml/min.

The P. angusta and S. cerevisiae F-ATPase inhibitor complexes were released F-ATPase inhibitoricomplexesarose column by applying a gradient of imidazole from 25 to 500 mM.

LC-MS separations were achieved with an Alltech Altima C18 column (2.1×100 mm, 3 µm) applying a gradient mobile phase of 40∶60 to 95∶5 acetonitrile∶water with 0.01% acetic acid throughout.

The selected method uses a Suplex pKb-100 HPLC column and applying a gradient with a mixture of acetonitrile, tert-butyl-methyl ether and water as mobile phases.

Native oligosaccharides and oxidized sugars (4-ketoaldose) were separated by applying a gradient with increasing concentration of sodium acetate over time as described by Westereng et al. [ 34].

The separation was performed at a flow rate of 150 nl/min by applying a linear gradient of 3%to40%0% B for 50 minutes.

The column was rinsed with 100 mL of SAB and subsequently eluted by applying a linear gradient of 0 to 500 mM NaCl in the buffer (100 mL) at the same flow rate.