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The fact that the His at the N-terminus of the LF-HMA is classified as a destabilizing residue compared to Ala, at least in Saccharomyces cerevisiae [31] and in Vero-Dr22 cells [32], suggested that the N-end rule might apply to LF stability in cells.
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These data clearly support the hypothesis that the N-end rule applies to LF potency in cells, likely by controlling cytosolic stability of the toxin.
Previous studies had provided evidence that the N-end rule applied to proteins containing the N-terminal LF domain [19], and since recognition of proteins by the ubiquitinating enzymes typically depends on a small region near the N-terminus of proteins, it was expected that the N-end rule would also apply to native, full-size LF.
The above frequency subband decomposition only applies to the Smt-LF mapping.
The newly developed method to estimate asphalt viscosity and aging trends using Low Field Nuclear Magnetic Resonance (LF-NMR) was applied to characterize the effect of incorporating recycled materials and recycling agents in asphalt mix samples.
Low frequency electromagnetic fields (LF-EMF) were applied to a subset of hearts perfused either aerobically (n = 9) or subjected to I/R (n = 9).
A dilution series of TEM8 and its mutants, combined with PA proteins (100 ng/ml) and LF (100 ng/ml), was applied to the cells to a final volume of 100 µl/well.
With its specific binding, Lf-R has been applied to gene delivery successfully, transferring genetic material to the hepatocytes [ 59].
In this article, we present the off-lattice single-cell-based hybrid model MultiCell-LF and discuss how this model can be applied to various aspects of tumor heterogeneity, both on cellular and microenvironment level.
It has been shown that H LF-NMR spin-spin (T2) relaxometry can be applied to differentiate between morphological populations in complex systems [ 8– 12].
Though hybrid methods produce better results, this study employed CS-LF to determine first its potential in finding solutions for the problem, particularly when applied to a real-world problem.
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CEO of Professional Science Editing for Scientists @ prosciediting.com