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The main reason is that its governing body, the Central Council of Church Bell Ringers, doesn't want to apply: "The primary object of the council is to promote and foster the ringing of bells for Christian prayer, worship and celebration," it said.
We apply the primary matching criteria defined in the task, which otherwise require event structures to be identical but include the approximate span and approximate recursive relaxations to exact match: the former allows small variation in predicted event trigger spans and the latter permits differences in the secondary arguments of recursive event structures for matches.
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By applying the primary mask, we expect to detect the signals of interest along with the interfering signals.
In our EAF-MR optimization method, we applied the primary algorithmic structure of the multi-resolution optimization, and we utilized the calculated SINR in order to reduce the number of configurations to be simulated.
Again, this is a more restrictive reading of the confrontation right than that of many lower courts which have rigorously applied the primary purpose test to doctors, nurses, social workers, and occasionally, teachers.
Before applying the primary antibody, sections were microwaved for antigen retrieval in 10 mmol/L citrate buffer (pH 6.0) for a total of 25 min, followed by equilibration in phosphate-buffered saline.
Negative control studies were performed without applying the primary antibodies.
Negative controls consisted of staining without applying the primary antibody and staining of a known INI1 negative AT/RT.
This difference is likely to be a result of the more stringent way in which we applied the primary care setting criteria.
The cryosections were blocked with 1% BSA in PBS with 0.1% triton for 1 h at room temperature before applying the primary antibodies.
After 15 min of blocking with normal serum (Invitrogen), we applied the primary antibody or corresponding control isotype IgG and incubated overnight at 4°C.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com