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Therein it was feasible to apply the assembly mode, as the 100 bp read length is significantly longer than the 37 bp used in the HapMap RNAseq data.
When using HLAminer to predict HLA types, we first trim the intronic portions and then apply the assembly mode with a CDS reference.
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The stability of the assembly is a crucial factor for a successful outcome, and it is determined by the design of the fixator, the screws and the spatial arrangement in which the surgeon applies the assembly.
BS, AP and US developed and applied the assembly pipeline.
We trimmed each sequence and applied the assembly method described above.
We applied the transcriptome assembly program Trinity to the assembly of a high quality reference transcriptome for North American ginseng.
In fact, many existing methods for de novo transcriptome assembly, like genome assembly, apply the de Bruijn graph approach with fragments of transcripts being simple paths in graph, in which a vertex is a k-mer and an edge exists between two vertices u and v if u and v appear consecutively in a read.
A porous electroactive composite based film made of core@shell nanospheres is uniformly fabricated on the conductive substrate by artfully applying the alternative assembly of the polyaniline/polystyrene (PANI/PSS) and the high-crystalline protonated Zeolite Socony Mobil-5 (HZSM-5) nanospheres via a potential pulse method.
In this paper, we consider the part flow in a high-quality relay production system applying the corrective assembly approach by incorporating machining, measurement and reprocessing errors simultaneously, and formulate the production rate of high-quality products satisfying the predetermined assembly tolerance.
We have applied the Rnnotator assembly pipeline to two yeast transcriptomes and compared the results to the reference gene catalogs of these organisms.
For each dataset, we used Tophat [ 15] tool to map short reads to the hg19 reference genome and applied the transcript assembly tool Cufflinks [ 16] to estimate expressions for all transcripts with known UCSC transcript annotations [ 17].
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