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We used it with some adaptations to our circumstances: we did not apply significance testing but selected 6 plaids for illustration.
Thus the ability to apply significance of predicted functional output no longer rests upon individual factors but on co-presentation and coherent regulation of these factors, reflecting the coordinated, interconnected nature of metabolic pathways themselves.
The low power issue is amplified by the need to apply significance thresholds derived from Bonferroni correction of billions of multiple tests with consensus thresholds (like 5.0E−08 for GWAS) not yet available (20).
In this publication we apply significance analysis to the spot-clusters of genes identified by the SOM method using three alternative test statistics based either on FC-measures or on regularized Student's t-statistics with special emphasis on the error characteristics of microarray expression data.
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Relative quantification [ 60] of miRNA expression levels derived from profiling experiments was tested for significant differences by applying significance analysis of microarrays (SAM) [ 61] which uses permutation.
Therefore, the sum of the variance weighted squared residuals (h) need to be below 5.99 to exclude significant measurement errors (with an applied significance level of α = 0.05).
We applied Significance Analysis of Microarrays (SAM) [97] to identify statistically significant genes in each case.
Post hoc analysis with Wilcoxon signed-rank tests was conducted with Bonferroni correction applied (significance level set at P < 0.0125) to test for significant difference in perceived safety of probiotics compared to other foods/products.
The significant differentially expressed genes were identified for multiple comparisons after applying significance cut-offs (adjusted p-value ≤ 0.05 and absolute (fold-change) ≥ 2, or ≤ 2).
Such research is often focused on testing theories that may have applied significance.
This investigation has applied significance because it attempts to ascertain the factors that will exacerbate misinformation acceptance as opposed to rejection.
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