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The ability to lock cells with guards adopting a "knock first" policy does not apply overnight and can be overruled at any time if there is an incident or a search.
Based on these results, we decided to apply overnight incubation as an average time to all the successive adsorption experiments to make sure that we always worked within the equilibrium regime.
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Primary antibodies were applied overnight at 4 °C followed by incubation of appropriate secondary antibodies conjugated with fluorophores.
Instead, XP awarded is stocked up, and only applied overnight while characters are resting, either in a paid-for hotel or for free in a campsite.
The same margin applied overnight Saturday into Sunday – in the US's favour – at Medinah in 2012, when José María Olazábal oversaw a recovery for the ages.
Anti-mouse HIF1α (0.5 μg/ml, AF1935, R&D Systems, Abingdon, UK) or HIF2α (1 μg/ml, AF2997 R&D Systems) was applied overnight at 4 °C.
For visualization of MCETs, primary antibodies directed against human mast cell tryptase (clonal AA1, mouse, DAKO) and C. albicans antibody (mouse monoclonal, ProSci) diluted in blocking solution were applied overnight at 4 °C.
Then the solution was heated to 65°C for 3 h with the permanent stirring (for better ligand exchange, stirring was applied overnight).
Freeze drying under vacuum was applied overnight to get the very dry gold nanoparticles, and then the samples were deposited on the surface of a KBr plate.
Primary antibodies were applied overnight at 4°C.
The primary antibody (AT8, Pierce; diluted 1∶2000 in TBS, 0.1% Triton X-100) was applied overnight.
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