CTCs found in peripheral blood might be apoptotic or viable but most currently applied assays do not discriminate this status.
These data show that the production of biodiesel and glycerol from prion contaminated MBM led to no detectable PrPsc or prion infectivity in the applied assays.
In our study we did see a surprising resilience of intestinal epithelial cells against DON when added to the apical side with virtually no effect in the applied assays.
This might be explained by the unequal sensitivity and specificity of the applied assays [ 31].
The 11 serum samples were titrated in a 2-fold dilution series in all applied assays.
The reproducibility of the applied assays was good, with a reproducibility of 85% and 88% for APC and K-ras, respectively.
Therefore, the discriminatory power of the applied assays is limited and other field isolates from this area may show identical characteristics (H. Tomaso, unpub. data).
The present simple qualitative assay using FeCl3 in well-cut diffusion assay is a cheap efficient alternate to the currently universal applied assay; Chromazurol-Shuttle assay.
However, considering the low concentration of Ficolin-1 in serum [33], [34] and the observation that rFicolin-1 did not interact with acBSA, it appears likely that Ficolin-1 does not mediate complement activation at the applied assay conditions.
However, when evaluating binding of the recombinant Ficolins added in equal concentrations, rFicolin-3 still appeared to have a better binding capacity to acBSA at the applied assay conditions compared to rFicolin-2.
