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Apart from that, all tools were applied with default settings.
Random forests and naive Bayes have been applied with default parameters (which is 100 trees for the random forest algorithm).
The prediction tools were applied with default parameters.
All parameters in MCODE are applied with default values.
iBBiG was applied with default parameters with nModules = 20.
cBowtie2 was applied with default options and –local mode.
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The Tophat algorithm (release 2.0.9, http://ccb.jhu.edu/software/tophat) was applied with defaults parameters to align all the reads onto the Aedes aegypti reference genome AaegL2.1 (http://ccb.jhu.edu/software/tophat) by taking into account, both already known (--no-novel-juncs) and novel ab initio splice exon-exon junctions [ 48].
'Singular Enrichment Analysis' was applied with the default parameters.
The algorithm was applied with the default settings of Weka version 3.6.0 [ 26].
A step size of 1 and window size of 10 were applied, with otherwise default settings.
We applied MutSigCV with default settings on somatic mutations identified by VarScan2 and muTect with and without the somatic RRA variants detected by RAREVATOR.
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