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(b) The final images from the 3-step filter applied to the template and search boxes respectively.
When an ac voltage (typically, 7 V rms, 10 kHz) was applied to the template electrode, the induced dielectrophoretic force repelled the cells from the electrode and pushed at the glass substrate to form the cell pattern which reflected the electrode shape.
Previously described primers, obtained from Integrated DNA Techs, USA, for detection of the four virulence genes were applied to the template [ 8, 14, 15] (Table 1).
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Shoulder width, in contrast, is extracted indirectly from the scaling parameter applied to the thorax template by the template-matching algorithm and not by detecting the humeral bristles, which are the landmarks used for manual phenotyping.
The different definitions of SW between the automated and manual method is a further plausible reason for the low observed correlation: the automated SW measure is calculated from the scaling parameter applied to the thorax template by the template-matching algorithm, whereas the manual procedure uses the humeral bristles as reference points.
GM masking was applied to the AAL template.
For each scan, the virtual screw coating was registered to the segmented coating and the resulting transformation was applied to the ROI template to generate the final ROIs for analysis.
Different growth conditions were applied to the GaN templates that had been treated with SiN x deposition and HF etching, and the same InGaN QWs (6 nm GaN/2.5 nm InGaN/10 nm GaN) were grown afterwards.
Degenerate primers targeting the gene from Arabidopsis thaliana encoding cytochrome P450 monooxygenase (Additional file 2) were applied to the cDNA templates.
In the < eavAttributeField > node, the attribute "name" specified the full path of one data field in the source data item and the value is the full path of the target data item Finally, the ARM mapping rules are applied to the archetypes, templates, and ARM constraints to generate the final relational database schema, as shown in Fig. 7.
In some instances, a constant potential of 0.4 V vs. Ag/AgCl was applied to the film for template extraction with ACN, thus facilitating the release of the template because of film swelling [ 130, 131].
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