Sentence examples for applied to the slice from inspiring English sources

Once per minute, five 0.5 ms pulses were applied to the slice at 40 Hz and recorded using AxoGraph X software (Berkeley, CA).

Gabazine was focally applied to the slice using a pipette.

All compounds were obtained from Sigma (Sigma Aldrich Canada, Oakville ON) or Tocris (Cedarlane Laboratories, Burlington ON) and stored in stock solutions at −20°C before being diluted and applied to the slice in oxygenated ACSF.

Yellow laser illumination applied to the slice (probably illuminating both the recorded neuron and surrounding axons) from 100 ms before to 50 ms after the electrical stimulation (Fig. 2B), clearly reduced the amplitude of the evoked EPSCs (Fig. 2D1, middle).

Pharmacological agents were added to the aCSF at the concentrations stated and applied to the slice via the perfusion system.

In interleaved experiments, vehicle (DMSO) or GSK-5 (500 n m) was applied to the slice for 20 min prior to LTP induction.

Meanwhile, the remaining n p =n b −n q unreliable users are applied to the slicer Q directly, as described by hat{b}_{textbf{t}_{p}(t)}(i =Q u_{textbf{t}_{p}(t)}(i)), {text{for}} tin [1,2,ldots,n_{p}], (27).

Insulin (0.5 100 nM) was dissolved in ACSF and applied to the slices for 1 3 hours.

Immediately after the slice preparation, fibrillar Aβ (1-40) was persistently applied to the slices at a concentration of 1 µM.

To exclude any potential side effects of bicuculline, picrotoxin (n = 4 slices), another blocker of GABAA receptors that does not block the SK channels, was applied to the slices.

After equilibration, the TUNEL solution was applied to the slices for 90 min at 37 °C.