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The optimization method is applied to the alignment and orientation of carbon monoxide to demonstrate its effectiveness.
The default arguments of BWA were applied to the alignment.
The same software and cost-set was also applied to the alignment of 18S rDNA.
Finally, the algorithm is not alphabet specific and can therefore be applied to the alignment of protein sequences.
The NovoCorrector was then applied to the alignment file produced to perform variant call and to produce error-corrected PacBio reads.
Therefore, it can only be applied to the alignment of sequences where the SV size is large enough for the algorithm to work in the non-classical mode.
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Codon substitution models [ 41] M1a M2a, M3a, M7, M7, and M8 were applied to the alignments.
Interestingly, this was achieved without any modifications to the search algorithm itself, since the score dissection was applied to the alignments post mortem.
Codon substitution models [ 71] M1a M2a, M3a, M7, M7 and M8 were applied to the alignments and these models assume variation in ω among sites.
Finally, the resulting amino acid sequences were aligned with EXPRESSO3D or TCOFFEE (the former was used when structural information could be applied to the alignments, otherwise the latter was used [ 55, 56]).
When applied to the alignments without alphabet reduction, the recommended steps identify 0.947 as the optimal value for myosin, and 1.0 as the optimal value for myoglobin (meaning there are not enough sequences for the analysis).
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