Sentence examples for applied to calculate the relative expression from inspiring English sources
The 2−ΔΔCT equation was applied to calculate the relative expression [ 23].
The 2−ΔΔCt equation was applied to calculate the relative expression of B-ALL samples [ 23].
The equation ratio 2−ΔΔ C τ was applied to calculate the relative expression level of miRNAs and targets using 5.8S rRNA and Actin gene as the reference gene, respectively.
After determination of the threshold cycle (Ct) for each LCM-derived aRNA sample, the PFAFFL method was applied to calculate the relative expression of each gene [ 75] using the 1 120 ovine fetal ovary cDNA dilution as calibrator sample.
The equation fold change = 2-ΔΔct was applied to calculate the relative expression of MGP in siRNA transfected glioma cells versus untransfected glioma cells or tumors versus normal cortex, respectively.
Delta CT method was applied to calculate the relative gene expression level [ 40].
After determination of the threshold cycle (Ct), the Pfaffl method [ 60] was applied as described in [ 59] to calculate the relative expression of each gene.
The 2−ΔΔCT method was used to calculate the relative expression levels of each gene.
To calculate the relative gene expression, the 2 −ddCt) method was used.
The dCT method was used to calculate relative gene expression levels as per protocol by applied biosytems.
The 2−ΔΔCt method was used to calculate relative gene expression.
