Sentence examples for applied to a column of from inspiring English sources

Cleared lysate was applied to a column of nickel sepharose resin (Qiagen).

Cleared lysate was applied to a column of glutathione sepharose resin (GE Life Sciences).

The crude protein was applied to a column of DEAE-Sepharose Fast Flow (2.0 × 40 cm).

Active fractions were collected and applied to a column of hydroxyapatite (3.0 cm i.d. × 15 cm length) equilibrated with 20 mM Tris HCl buffer (pH 8.0).

Each sample (2.0 mg) was dissolved in distilled water (2 mL), passed through a 0.45 μm filter, and then applied to a column of gel-permeation chromatographic at a flow rate of 0.5 mL min−1 [14].

The filtrate was applied to a column of Butyl-Sepharose High Performance (GE Healthcare Life Stiences, Chalfont St Giles, UK) equilibrated with 10 mM Tris HCl (pH 7.4) containing 25% saturated ammonium sulfate.

Biologically active fraction I.2 (6 g) was re-dissolved in a minimum volume of petroleum ether (P.ether):CHCl3 EtOAc mixture (2 2 1) and was applied to a column chromatography consisting of 800 g of silica gel.

The dialyzate was applied to a column (1.8 × 17 cm) of DEAE-Sepharose CL-6B equilibrated with 10 mM sodium phosphate buffer (pH 6.5).

Then it was applied to a column (1 × 17 cm) of CM-Sephadex C 50.

The collection with laccase activity was applied to a column (1 × 40 cm) of Sephadex G-75.

Precipitated material was removed by centrifugation at 800 g for 10 minutes and the supernatant was applied to a column (2.5 × 20 cm) of DEAE-Sephadex A 50.