Despite the different mechanisms of applying stringency, we found that a cation gradient behaved very much like a temperature gradient during assay development.
In this study, we applied two stringency control strategies for surface plasmon resonance (SPR) detection of DNA hybridization and discrimination of completely and partially complementary 24-mer sequences.
To more precisely compare the predictors, we have applied three stringency thresholds corresponding to the 99, 95 and 90% specificities, respectively.
Discrimination is achieved by applying thermal stringency to denature the mismatched reporters.
Applying a stringency gradient alone, resulted in 10 20% higher success rates (compare strategy 7 and 9).
Applying high stringency filters to the ∼2.1 million Draft SNP to improve their conversion rate.
A first filtering step was performed applying low stringency filters to four parameters.
When applying certain stringency thresholds, it also significantly outperforms PSI-BLAST and BLAST at nearly all of the thresholds (Table 2).
For statistical evaluation, different levels of stringency were applied so as to reflect stringency levels commonly found in the literature [8], [14], [16].
Phylogenetic footprinting was applied at different stringency, to allow the following optimization of the protocol (below).
