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We performed multi-cycle CPEC using identical conditions as described except that during the annealing step, we applied slow ramping at 0.1°C/second from 70°C to 55°C before annealing at 55°C for 30 seconds.
We then applied slow voltage ramps to inactivate transient sodium currents (I NaT ), thereby permitting isolation of I NaP.
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For the clusters without any BLASTP hits, we applied slower but more sensitive homology detection tools PDB-BLAST[31] and FFAS[33] to search for more remote homologs.
Samples were also synthesized using conventional calcination methods (applying slow heating and long exposure times, SHLE series).
People bristle and snooze, but digital is like a weapon and the old saying definitely applies – slow is smooth, smooth is fast.
A market survey has shown that 75% of the surveyed liners and carriers apply slow steaming in order to save bunker costs (Seatrade Global 2014).
There is a saying used by shooters that applies: "Slow is smooth, smooth is fast". In this context, it means that change management is vital to digital transformation, no matter how imminent the external threat.
Dynamic stretching, by contrast, involves taking a position at the end range of motion, applying slow, steady pressure to push that boundary and then returning.
Lysis was performed by applying slow strokes during maximally one minute.
For organs that rely on a large pool of stem cells, if an immortal strand strategy applies, slow cycling of cells at the top of the lineage hierarchy would be beneficial as the stem-cell pool expands during development [ 9], but once the stem-cell compartment is fully developed stem cells would cycle quickly.
Instead, apply slow, steady pressure until the knife breaks through the can.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com