Sentence examples for applied one hour from inspiring English sources

If required, an anesthetic cream was applied one hour before venipuncture.

For the identification of pathways regulating expression of candidate genes, the small molecule inhibitors AG1478 (20 μM), PP2 (20 μM), LY294002 (10 μM), or U0126 (10 μM), respectively, were applied one hour prior to hEGF stimulation.

Session 2 (Apply): One hour.

Slides were loaded on a DAKO autostainer and blocked with serum-free protein block solution (Dako #X0909) which was applied for 5 minutes and then FAK primary antibody (Millipore #05-537) wappliedied for one hour.

Primary antibodies were then applied for one hour at room temperature, and secondary antibodies (Alexa Fluor 488, 546 or 647, Life Technologies) were applied for 30 min at room temperature.

NPC was applied for one hour.

TUNEL reaction mixture was applied for one hour at 37°C.

After washing with PBS, secondary antibody was applied for one hour, followed by washing in PBS.

A 1 20 dilution of anti-IBAT protein antibodies was applied for one hour.

DMSO/BIRB796 (5 or 20 mg/kg BW) was applied intraperitoneally one hour prior to vessel clamping.

Secondary antibody was then applied for one hour at room temperature.