Sentence examples for applied on sections from inspiring English sources

Anti-digoxigenin antibody (Roche) was preadsorbed at 1∶1000 dilution in blocking solution for 2 hours and then applied on sections overnight at 4°C.

The primary 1/10 diluted polyclonal anti-IL-6 antibody (Genzyme Co., Cambridge, MA, USA) was then applied on sections of the colorectal carcinomas followed by a biotinylated secondary antibody.

Table 4 demonstrates examples of detailed examinations of safety effects of the road infrastructure improvements applied on road sections.

Figs. 2 3 provide a visual presentation of the significant results - the efficiency indices and their confidence intervals, for treatments applied on road sections and junctions/interchanges, respectively.

After blocking buffer was removed, primary antibody was applied on the sections.

Streptavidin conjugated with peroxidase was subsequently applied on the sections (DAKO, Glostrup, Denmark).

They were diluted in 1% NDS/1% BSA/TBS and applied on tissue sections for overnight incubation at 4 °C.

The primary mAbs (VIG8, 196X, GB24) were applied on the sections at 5 μg ml−1 and incubated overnight at room temperature.

Monoclonal 14-3-3σ 14-3-3σ 14-3-3σ 1433S01, 1:75, 2.7 μg IgG1/ml) from NeoMantibodyclone.S.A were applied on the sections for 30 min at room temperature.

After washing with Stop/Wash Buffer for 10 min, the secondary antibody anti-digoxigenin peroxidase conjugate was applied on the sections for 30 min at room temperature.

The antisense riboprobe diluted at 800 ng ml−1 in hybridisation buffer was denatured and 100  μl applied on each sections for an incubation of 8 h at 42°C.