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It is also still not clear, how this approach would best be applied in developmental toxicity, where the baseline of gene expression changes over time (Penschuk et al. 2006; Bal-Price et al. 2015; Smirnova et al. 2014).
Since the launch of this technique, OPT has been applied in developmental biology (10, 13, 23), for gene-expression analysis (25), to localize labeled cells within a tissue (30), and to screen normal and abnormal anatomy (12, 24).
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Because of the significant public health implications, the best science must be applied in assessing developmental toxicity data.
To determine if this compound exhibited antimicrobial activities against F. graminearum, serotonin was applied in vitro at different developmental stages of the fungus.
Advantages of this model over existing models of NEC include its simplicity and the fact that it can be applied in mice at a developmental stage that is relevant to human NEC.
It is recommended that the training applied in this research be implemented in developmental guidance programs.
This framework was applied in order to assess the developmental regulation of the two reference genes Actin11 and Elongation Factor 1-β in the apex of poplar root.
The ELDS, for example, can be applied in creating assessments of children's developmental progress.
Immunostaining for OR1 was applied in order to determine at what developmental stage the receptor is expressed and neurons could react to the OXA application.
However, we believe that similar approaches can be applied in other organisms, both in traditional developmental genetics systems (e.g. flies, mice) and nonstandard model systems (e.g. guppies, beetles).
The Institutional Review Board of the New York State Institute for Basic Research in Developmental Disabilities approved the methods applied in this study.
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