Sentence examples for applied immobilized from inspiring English sources

After successful purification and immobilization of folding catalysts, we applied immobilized mini-chaperone and foldases to refold the cysteine- and proline-rich proteins.

Aliquots of proteins in sample buffer were applied onto immobilized pH 3 10 non-linear gradient strips (immobilized pH gradient strips; Bio-Cyte, Hercules, CA and Bio-Rad, Hercules, CA).

A mixture of M13-Cry1Ac and M13-Cyt1Aa phages (1 1) was applied to immobilized cadherin fragment CR7-12 (1), anti-Cry1Ac antibody (2) and anti-Cyt1Aa antibody (3).

Samples (40 μg/IPG strip) were applied to immobilized pH gradient strips (7-cm, non-linear pH 3 10, Bio-Rad) for 1 h at room temperature.

After a nuclear extraction, the samples were digested with trypsin, and the resulting peptides were applied to immobilized metal ion affinity chromatography (IMAC) column to enrich for phosphopeptides.

Samples were subjected to two-dimensional (2D) electrophoresis as follows: the desalted proteins were dissolved in Zoom 2D protein solubilizer-1 and applied on immobilized pH 3 10 linear gradient strips according to the manufacturer's instructions (Invitrogen).

Samples of 250  μg protein were applied to immobilized (pH 3 10) nonlinear gradient strips (11 cm; Bio-Rad Laboratories).

Samples of 1 mg protein were applied on immobilized pH 3 10 nonlinear gradient strips in sample cups at their basic and acidic ends.

The resulting cell free extract was applied on immobilized Ni2+-column (His-Trap™ HP-column from GE-Healthcare) by employing an ÄKTAprime system.

Samples were applied to immobilized pH gradient (IPG) strips (pH 4 7 NL, 24 cm, from GE Healthcare, Munich, Germany) by in-gel rehydration.

In our previous research works, we immobilized a γ-lactamase (Gla) from Sulfolobus solfataricus fused with a His-tags on nickel-chelating agarose and applied the immobilized enzyme in batch reactions.