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Under the applied culture conditions, P. cinnabarinus SBUG-M 1044 produced an extracellular phenol oxidase with an activity of around 0.5 U mL-1.
Nevertheless, the applied culture methods have been adapted to isolate the common bacteria known to be involved in bronchial infections of CF patients.
These data demonstrate a differentiation of pmATII cells towards an ATI-cell-like phenotype under the applied culture conditions.
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To investigate the composition of organisms in this space, we applied culture-independent approaches to microbes harvested from the air of two densely populated urban buildings, from which we analyzed 80 megabases genomic DNA sequence and 6000 16S rDNA clones.
The applied culturing method proved to be suitable for the purpose of the current study.
To improve the reproducibility of high-throughput cultivation processes and the comparability between different applied cultures, it is strongly recommended to use automated or manual liquid handling stations or, alternatively, multi-channel pipettes.
This study showed that the conventional method of using 96-pin replicators to transfer E. coli PR02-cells from one MTP to another MTP can result in highly variable growth kinetics of the applied cultures.
The resulting cell suspension was applied to culture flasks and maintained in culture medium at 37°C in a 5%CO2/95%% air incubator.
Without systematic assays for optimizing hNSC culture conditions, conditions optimized for rodent cultures have been applied in culturing hNSCs, with disappointing and inconsistent results.
In addition, when we applied a culture score to describe bacterial loads in the lymph nodes cultures, we also observed a significantly lower bacterial burden (Table 1, p<0.01).
H. pylori culture suspension was used to inoculate the plates, and discs containing 1, 10, 100 μg of compounds were applied onto culture plates.
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