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High salinity stress was applied by watering soil-grown plants with 150 mM sodium chloride.
The propagation plugs for treated plants were first reduced to 60% saturation before the salt stress was applied by watering with 0.5× nutritive solution supplemented with 150 mM NaCl.
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This also reduced the transmission pressure applied by water buffaloes, given the 100∶1 buffalo to human stool weight ratio assumed by the model, resulting in the model making more conservative predictions.
Drought stress was applied by withholding watering starting from 14th day for all experimental groups, except the control group (Day 0).
When plants were 30 days old, corresponding to late vegetative stage, deficit irrigation treatments were applied by withholding watering on the stressed pots while controlled pots were water daily to soil capacity.
Note that this study focuses on water applied by irrigation (blue water), while water provided by rainfall (green water) is not considered.
Chakraborty and Pradhan (2012) experimented enzymatic performance of five different wheat varieties in response to 3, 6 and 9 days of drought stress applied by withholding watering in pots.
A slow water stress was applied by withholding water for 23 days starting at stage DC14-19 (Zadokscalele) and approximately till tillering (Zadoks stage DC22-29) when the final RSWC level was 27-28% which corresponds to a severe stress.
Drought stress was applied by replacing water with 20 % PEG solution and roots were totally covered by PEG solution [ 108, 109].
After applying salt stress by watering two-week-old plants with 100 mM NaCl twice in 3 days, the rosette diameter of soil-grown plants was measured.
The additional irrigation water requirement for each treatment was applied by adding surface water with EC of 0.5 dS m−1.
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