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For the vibrations of the ligands, a scaling factor of 0.96 was applied as determined by Wong [30] for B3LYP/6-31G B3LYP/6-31G assumption that the p-functions added to the H atoms do not significantly affect the vibrational frequencies of the C-C and C-O bonds.

For both ML and BA, the best-fitting model of evolution was applied, as determined by Modelgenerator V.85 following the Hierarchical Likelihood Ratio Test (with four discrete gamma categories).

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Bending was applied successively as determined by the degree of strain (ductility) of the femur by the Test Star software programme (Utvag, Tromsoe, Norway).

The protein loads used in the Western blot assays were chosen to be within the range that gave a linear correlation of band densities with amount of applied antigen, as determined in optimization experiments.

The constructs were then placed on a universal testing machine UTM (Fig. 1d), and increasing axial loads were applied to determine points of loosening as determined as mechanical failure on the load deformation curve.

Other terms apply as well.

fluorescent probes have been applied as labels to determine functional groups in polymeric materials.

In the present study, partial derivatives were applied as an aid to determine the exergoeconomic optimum of the HACHP, which proved to be a helpful tool.

Therefore, the number of flexible bonds in the shortest path (as applied to classify the atom-pairs) can also be applied as a heuristic to determine the number of Gaussian components for the GMM.

At the end of the experiment, AMPA and GABA receptor antagonists were bath applied as described above to determine the nature of the local synaptic inputs.

For the BA, a different model was applied for each gene as determined by Modelgenerator.

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