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Applied to raw intensities, the quality metrics can help with monitoring experimental procedures and with the choice of normalization procedure; application to the normalized data is more relevant for assessing the utility of the data in downstream analyses.
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The current amplitude during the drug was normalized to that before the drug application, and the normalized values were used to plot the dose-response curve.
Finally, we use a simple but explanatory example in requirements engineering to illustrate the application of the normalized framework.
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