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We demonstrated fast, reliable genotyping of five SNP markers in methylenetetrahydrofolate reductase (MTHFR) gene, known to be associated with hyperhomocysteinemia and cardiovascular diseases, using RFMP assay and also assessed the potential for application to determination of allele frequencies in DNA pools.
Using RFMP assay, we demonstrated highly reliable genotyping of five SNP markers in MTHFR gene, known to be associated with hyperhomocysteinemia and cardiovascular diseases, and also provided the potential for application to determination of allele frequencies in DNA pools as a means of efficiently screening SNPs and prioritizing them for further study.
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Our objective was to determine whether the degree of variability associated with dermal microdialysis allows its practical application to determinations of bioequivalence of topically applied agents with a reasonable number of subjects.
The proposed electro-fluorescence sensor has a potential application to the determination of Fe3+ in environmental and biological systems.
The design and characterization of a lactate biosensor and its application to the determination of this analyte in wine and beer are described.
A chemosensor based on rhodamine B has a good potential application to the determination of Cu2+ in environmental and biological systems.
These results will be the basis for the main experiment with application to quantitative determination of adulterated skimmed milk (Part II).
The experimental equipment for the measurement of α-particle energies is described together with its application to the determination of the position, with respect to the oxide surface, of a layer containing a small concentration of radon atoms.
In the next section we define the model and follow this with a specific application to sex determination; further details of the simulations are given in the Methods.
Although useful for the determination of molecular-weights of proteins, its application to the quantitative determination of protein concentrations is more difficult.
Applications to the determination of the initial configuration of the unit cell of a typical example of a 5-layer angle-interlock fabric, and to the simulation of a transverse compression test and a forming test are presented.
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