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In experiments with toxins, 0.1 mg/ml bovine serum albumin (BSA, Sigma) was added to the application solution to prevent non-specific binding.
In experiments where the Ba2+ concentration was lower (3 mM) and/or was replaced with Ca2+ (2 mM), the equivalent amount of NaCl was added to the application solution to preserve osmotic pressure (TTX, tetrodotoxin, Alomone Labs, Jerusalem, Israel; TEA, tetraethylammonium, Sigma; 4-AP, 4-aminopyridine, Merck).
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For photogrammetric applications, solutions to camera exterior orientation problem can be classified into linear (direct) and non-linear.
The chapter concludes with the presentation of two practical applications, the solution to a truss and an electrical circuit problem.
To mitigate this behaviour, we propose an application-level solution to balance the stress and put the timing-critical gates of the critical path into a relaxed (balanced) mode.
Although K+ blockers were added to the application solution, the recording was contaminated by voltage-gated K+ currents (Fig. 1b).
After blocking each sub-type channel we exposed the patch to an application solution containing 50 µM Cd2+.
To test this possibility we measured the steady-state inactivation of the current when the R-type blocker, SNX-482 (30 nM) was added to the application solution.
To isolate the Q-type current the blockers for L-, N-, R- and P-type channels were added to the application solution before blocking the Q-type channel (Fig. 5e).
But it left the practical application of a solution to the groups involved.
Application of the solution to cases under investigation with ECoG has also been effected to demonstrate its utility.
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