Therefore the application of this assay is rather limited.
Several experimental factors of difficult control, such as variation of cell density, hinder a reliable and reproducible application of this assay.
However, method validation predictions of the quantitative performances of the assay and estimations of measurement uncertainty may be far away from the real performances obtained during the routine application of this assay.
As the method is designed directly towards the cellular function of MTH1, activity of MTH1 in different breast cancer cell lines has been detected, implying the potential application of this assay method for biomedical research and clinical diagnose in the future.
This phenomenon has negated the application of this assay as a bona fide stem/progenitor cell assay, since these cells seem to lack self-renewal activity.
In the beginning of the application of this assay, sequencing validated mutant samples were included in the runs as controls for the amplification of the mutant allele.
Furthermore, with the application of this assay, we identified 28 natural product candidates.
To show the application of this assay with physiological samples, the kinase activity experiments were repeated with diluted HepG2 cell lysate.
