Sentence examples for application of the secondary from inspiring English sources

Neurite extension was determined by primary antibody (beta-III-tubulin) application for 1 h, three 1 min washes in PBS, and then application of the secondary antibody (AlexaFluor 488) for 30 min.

After application of the secondary antibody, cells were washed with PBS, and FISH was performed.

Following the application of the secondary antibody the substrate chromogen solution (DAB) was added as a visualisation reagent.

Then slides were washed (three changes over 1 h) in PBS containing 0.1% Tween 20 (PBS/Tween), before the application of the secondary biotinylated antibody (DAKO, Kyoto, Japan).

After application of the secondary antibody, biotinylated rabbit anti-mouse (DakoCytomation, Ely, UK), the antigen was visualised using diaminobenzidine (DakoCytomation) and the sections were counterstained with haematoxylin, dehydrated and mounted.

Sections were washed, and after application of the secondary antibody (R&D Systems), tissues were developed using Vectastain ABC (Vector Labs, Burlingame, CA, USA) and 3,3′-diaminobenzidine (Vector Labs).

The present paper aims to propose an original steam generation system, which resulted suitable for the application to the secondary loops of lead-cooled fast reactors: reaction heat is extracted from the lead by means of a coolant under vapor phase, thanks to both an external feed-water boiling and special bayonet tubes in the primary secondary fluid heat exchangers.

Additionally, pre-absorption of little SAAS antibodies with little SAAS peptide or application of only the secondary antibody does not result in immunostaining of rat hypothalamic tissue.

Apart from the application of the predicted secondary structure, hydrophobicity, van der Waals volume, polarity, polarizability, and different dimensions of pseudo-amino acid composition vectors in similar studies, the position specific scoring matrix (PSSM) has also been used to improve the correct classification rate (CCR) in this study.

Sections were washed in PBS 3 × 5 min, before application of the biotinylated secondary goat antibody (Vector Laboratories, Burlingame, CA, USA) diluted 1 : 200 for 40 and 30 min, respectively.

After stringent washing with high salt TBS and 0.6% Tween 20 (BioRad Laboratories, Hercules, CA, USA), detection of positive binding was performed by the sequential application of the appropriate secondary antibody diluted in non-immune block and avidin biotin peroxidase conjugate (Dako, Glostrup, Denmark).