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The practical application of the probe was successfully tested in the human blood and urine samples.
Application of the probe for selective detection of fluoride was demonstrated by live-cell imaging.
Fluorescent imaging inside HepG2 cells indicated potential application of the probe for detecting Zn2+ ions in living cell imaging.
Moreover, cellular application of the probe was successfully performed for the mitochondrial imaging of HepG 2 cells.
And the cell imaging experiment indicated a successful application of the probe R1 for Fe3 + in living cell.
It is noteworthy that the low cytotoxicity, the intrinsic aggregation-induced emission nature and near-infrared emissions enable the application of the probe in living cell imaging.
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The application of the probes in detecting N2H4 in living cells and in the gas state was also investigated.
Application of the probes to an additional group of 100 clinical isolates revealed that 87% of Gram-positive anaerobic cocci (GPAC) could be identified with this set of probes.
To confirm the practical application of the probes, a water sample from a lake was filtered through a 0.2-μm membrane.
The application of the probes 3– 13 for the investigation and exploitation of other PKS systems will be reported in due course.
Perhaps the two most promising aspects of structural probing for RNA engineering are the adaption of these techniques to high-throughput sequencing platforms and the improvements in the chemistry and range of application of the probes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com